Fig. 1. Adipogenic differentiation affected by glucose and glutamine content. Quantitative ORO staining of lipid content in adipocytes differentiated either in medium with no glucose (0 mM), low glucose (5.5 mM) or high glucose (25 mM). Ctrl (untreated cells in ST medium); AD+Glutamine (adipogenic medium supplemented with 5 mM glutamine); AD+MSO (glutamine-free adipogenic medium in conjunction with irreversible GS inhibitor methionine sulfoximine (MSO)). Data expressed with arbitrary units analysed using one-way ANOVA, followed by Tukey's post-hoc test and shown as mean ± SEM. Statistical significance set at p < 0.05, n = 3.